How many peridinin & chlorophyll are in a PerCP complex?

The PerCP complex has a high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PerCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PerCP complex is a dimer with a molecular weight of approximately 35kDa.

What is PerCP used for in research applications?

PerCP is commonly used in research applications such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in these techniques. Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

What is a PerCP conjugate?

PerCP conjugates are large complexes suitable for cell surface labeling techniques such as flow cytometry. PerCP is available conjugated to:

What is a PerCP conjugate in Dinophyceae SP?

Jackson ImmunoResearch offers the form found in Dinophyceae sp. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission peak at 677 nm. PerCP conjugates are large complexes suitable for cell surface labeling techniques such as flow cytometry.

What is the spectra of PerCP conjugated to an affinity-purified secondary antibody?

PerCP has a broad spectrum of excitation with a main peak at 472-483 nm, and a long Stokes shift to an emission peak at 677 nm (Figure 1). Figure 1. Relative shape and position of spectra in the peak region of excitation (blue) and emission (purple) for PerCP conjugated to an affinity-purified secondary antibody .

What is a good alternative to PerCP eFluor?

PerCP exhibits a large Stokes' Shift, with an excitation peak at 482 nm and an emission peak at 675 nm. PerCP is was historically used in flow cytometry, however it is highly susceptible to photobleaching and has poor stability. Alternatives like BB700, NovaFluor Blue 690 or PerCP-eFluor™ 710 are preferred.